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Foxa1 gene and protein in developing rat eccrine sweat glands

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Abstract

To investigate the development of eccrine sweat glands and the expression of Foxa1 genes and proteins in the course of development, the footpads from E15.5 to E21.5, P1–P12, P14, P21, P28 and P56 rats were subjected to immunofluorescence staining of FoxA1 and double immunofluorescence staining of K14/α-SMA, FoxA1/K7 and FoxA1/α-SMA, and were processed for Foxa1 gene detection by RT-qPCR. The results showed that rat eccrine sweat gland germs was first observed emerging from the basal layer of epidermis at E19.5, and then elongated downward into the dermis, forming straight ducts by E21.5. Early development of the secretory segments appeared at P1. The Foxa1 gene was not expressed in rat footpads until P2, but from P2 to P5, its expression up-regulated sharply, and thereafter maintained at a high level until adulthood. FoxA1 protein was first observed at P6 in eccrine sweat glands, four days after initial detection of Foxa1 gene transcripts. In skin, FoxA1-positive cells were present exclusively in secretory coils, with 95% being K7-positive secretory cells and 5% being α-SMA-positive myoepithelial cells. We conclude that Foxa1 can be used as a marker of eccrine sweat glands in skin and also as a marker of secretory coils, and Foxa1 is related to the development of secretory coils.

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References

  • Cui CY, Childress V, Piao Y et al (2012) Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na–K–Cl cotransporter 1. Proc Natl Acad Sci USA 109:1199–1203

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Friedman JR, Kaestner KH (2006) The Foxa family of transcription factors in development and metabolism. Cell Mol Life Sci CMLS 63:2317–2328

    Article  CAS  PubMed  Google Scholar 

  • Hannenhalli S, Kaestner KH (2009) The evolution of Fox genes and their role in development and disease. Nat Rev Genet 10:233–240

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kunisada M, Cui CY, Piao Y, Ko MS, Schlessinger D (2009) Requirement for Shh and Fox family genes at different stages in sweat gland development. Hum Mol Genet 18:1769–1778

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li H, Li X, Zhang M et al (2015a) Three-dimensional co-culture of BM-MSCs and eccrine sweat gland cells in Matrigel promotes transdifferentiation of BM-MSCs. J Mol Histol 46:431–438

    Article  CAS  PubMed  Google Scholar 

  • Li H, Zhang X, Zeng S et al (2015b) Combination of keratins and alpha-smooth muscle actin distinguishes secretory coils from ducts of eccrine sweat glands. Acta Histochem 117:275–278

    Article  CAS  PubMed  Google Scholar 

  • Li H, Zhang M, Chen L, Li X, Zhang B (2016a) Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice. J Mol Histol 47:485–490

    Article  CAS  PubMed  Google Scholar 

  • Li M, Zhang W, Liu C et al. (2016b) Forkhead box A1 (FOXA1) tagging polymorphisms and esophageal cancer risk in a Chinese population: a fine-mapping study. Biomark Biochem Indic Expo Response Susceptibility Chem 21(6):523–529

    Google Scholar 

  • Liu Y, Zhao Y, Skerry B et al (2016) Foxa1 is essential for mammary duct formation. Genesis 54:277–285

    Article  CAS  PubMed  Google Scholar 

  • Pristera A, Lin W, Kaufmann AK et al (2015) Transcription factors FOXA1 and FOXA2 maintain dopaminergic neuronal properties and control feeding behavior in adult mice. Proc Natl Acad Sci USA 112:E4929–E4938

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saga K (2002) Structure and function of human sweat glands studied with histochemistry and cytochemistry. Prog Histochem Cytochem 37:323–386

    Article  CAS  PubMed  Google Scholar 

  • Wang W, Yi M, Chen S et al (2016) Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells. Oncotarget 7:16745–16759

    PubMed  PubMed Central  Google Scholar 

  • Whitington T, Gao P, Song W et al (2016) Gene regulatory mechanisms underpinning prostate cancer susceptibility. Nat Genet 48:387–397

    Article  CAS  PubMed  Google Scholar 

  • Zhang G, Zhao Y, Liu Y et al (2016) FOXA1 defines cancer cell specificity. Sci Adv 2:e1501473

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This manuscript was supported in part by the National Natural Science Foundation of China (81471882, 81071551), and the Natural Science Foundation of Guangdong Province, China (2014A030313476).

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Authors and Affiliations

  1. Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China

    Haihong Li, Liyun Chen & Mingjun Zhang

  2. Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China

    Haihong Li & Bingna Zhang

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  1. Haihong Li
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  2. Liyun Chen
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  3. Mingjun Zhang
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  4. Bingna Zhang
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Correspondence to Haihong Li.

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Li, H., Chen, L., Zhang, M. et al. Foxa1 gene and protein in developing rat eccrine sweat glands. J Mol Hist 48, 1–7 (2017). https://doi.org/10.1007/s10735-016-9700-5

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  • DOI: https://doi.org/10.1007/s10735-016-9700-5

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